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I assume you have the x16 riser (5C50W00933)? See my posts on previous page, but essentially this riser only seems happy with x8 and x16 cards. If you want to use an x1 or x4 card you need the 5C50W00909 riser. This resolved my issue.

Unfortunately the workaround did not work. I did have success with an x8 card but for my x4 card I think I'll have to get the 5C50W00909 riser. Working: Intel X520-DA2 PCIe x8 (E10G42BTDA) Not Working: StarTech I225-V PCIe x4 (PR42GI-NETWORK-CARD) Dynamode USB 3.0 PCIe x1

I've got a I225-V x4 card that is not detected (works in another system) so I'm going to try the workaround in this post when I get chance.

Can confirm there is continuity between B31 and B81.